Cut flowering chrysanthemum: varietal improvement interventions (CRIREC)
RDP 2014-2020 Measure 16.01
Goals
Recover, rehabilitate and make available to Ligurian growers varieties of cut flowering chrysanthemums that are very commercially attractive but have now disappeared from the catalogs of both Dutch and Danish breeders.
Application no.
12834
Members
Florcoop Sanremo sca
IPSP-CNR di Torino
CREA-OF di Sanremo
Project duration
01/03/2021 – 30/11/2022
The project
Examples of chrysanthemum varieties that have disappeared from the catalogs of major breeders include Dilana, Snowdon, and Turner, which are still in demand in the domestic market but have lost the variety’s inherent positive characteristics due to viral diseases spread by continuous agamic propagation.
To restore these varieties, sampling was conducted for:
- Diagnosis of viruses and viroids with Next Generation Sequencing techniques to recognize known and novel viral forms with molecular diagnostic assays for control of rehabilitated plants
- Taking plant material for remediation. Plants were placed in vitro, restored by heat treatments, and following meristem collection were micropropagated in vitro. Restored plants were set in a greenhouse with insect proof net to have a pool of mother plants:
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- Checked for the absence of viruses
- For the production of agamic material to meet territorial needs
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Download the slides produced for the International Society for Horticultural Science (ISHS)
Download the slides from the December 3, 2021 seminar of CREA-OF in San Remo
Download the slides from the December 3, 2021 seminar at CNR-IPSP Turin
Download slides on rehabilitation of virus-infected chrysanthemum varieties from the July 29, 2022 practical demonstration
Download the slides from the November 04, 2022 CREA-OF seminar in San Remo
Download the slides from the November 04, 2022 seminar at CNR-IPSP in Turin, Italy
Download Protected Crops article, “Chrysanthemum, the sector can restart in Liguria.”
Download the final project brochure
Download the poster of the presentation at the AKIS conference on October 26, 2023
Development of an NGS diagnosis system
Development of an NGS diagnostic system capable of obtaining information on the viral/viroid infection status of chrysanthemum varieties important to western Liguria and a protocol system for the rehabilitation of endangered varieties.
Practical demonstration 29/07/2022.
Results of virological analysis and remediation performed on cut flowering chrysanthemum varieties
The video shows the results obtained as a result of the application of NGS techniques on cut flowering chrysanthemum varieties of particular interest to western Liguria such as Turner, Dilana and Snowdon highlighting new viruses and a viroid present in the material and, after remediation, the availability of pink Dilana material completely virus-free and therefore ready to be propagated and distributed to Ligurian flower growers
Seminar 04/11/2022.